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polyclonal anti hbs  (Novus Biologicals)


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    Novus Biologicals polyclonal anti hbs
    (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
    Polyclonal Anti Hbs, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti hbs/product/Novus Biologicals
    Average 95 stars, based on 45 article reviews
    polyclonal anti hbs - by Bioz Stars, 2026-06
    95/100 stars

    Images

    1) Product Images from "Adenine Base Editing Potently Suppresses Hepatitis B Surface Antigen Expression and Inhibits Hepatitis D Virus Release"

    Article Title: Adenine Base Editing Potently Suppresses Hepatitis B Surface Antigen Expression and Inhibits Hepatitis D Virus Release

    Journal: bioRxiv

    doi: 10.64898/2026.02.06.704371

    (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.
    Figure Legend Snippet: (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Control, Western Blot, Staining



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    (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
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    Image Search Results


    (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.

    Journal: bioRxiv

    Article Title: Adenine Base Editing Potently Suppresses Hepatitis B Surface Antigen Expression and Inhibits Hepatitis D Virus Release

    doi: 10.64898/2026.02.06.704371

    Figure Lengend Snippet: (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.

    Article Snippet: Immunodetection was performed using primary antibodies including monoclonal anti-HBs (Abbott H166 mouse), polyclonal anti-HBs (Novus biologicals NB100-62652 rabbit), anti-Ku80 (ab119935, mouse, Abcam), anti-HDV (homemade, mouse), followed by incubation with horseradish peroxidase (HRP) or fluorophore-conjugated secondary antibodies.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Control, Western Blot, Staining